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KMID : 0378019630060110035
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New Medical Journal
1963 Volume.6 No. 11 p.35 ~ p.43
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Histological Study of the Preserved Bones Using Different Methods of Preservation
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Abstract
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1) A study has been made on determination of types of preserved bore in order to keep them as fresh as possible for a long period of time, using rabbit bones with different preserved solution and method, particulary with different preservation temperature.
2) As preserved solution we used saline,5 % dextrose,Ringer´s solution, merthiolate,cod liver oil, auto-serum, hetero-serum, citrated auto-blood, citrated hetero-blood and 70% alcohol. According to different solution, bone was kept 2, 4, 6, 12 weeks or 3, 6, 15, 24 weeks in each solution and then was fixed in 10% formalin for 48 hours, decalcified in 5% nitric acid and slide was made from paraffin section. After the slide was stained with ordinary H & E stain, a microscopic examination was made.
3) With refrigeration method, they bones preserved in auto-serum, merthiolate and saline showed relatively fresh state until 2 to 3 weeks but after 3 weeks those preserved in merthiolate and saline showed reticular structure became cracked and degenerative change of the cells of the bone cortex and marrow. Those preserved in cod liver oil, citrated blood and hetero-serum, showed already degeneration, necrosis and disappearance of the cells of the bone cortex and of the marrow and the nuclei displayed pyknosis.
4) Using deep freezing method, the nuclei of the marrow cells showed only slight pyknosis and general structure of the bone was preserved relatively well until 6 weeks. No significant degeneration change was noted in the structure and staining reaction until the 24 th weeks, using this method.
5) For preservation of bone for short period of time, auto-serum, merthiolate and saline are suitable. For long preservation deep freezing method is ideal.
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